Zebrafish p53 protein enhances the translation of its own mRNA in response to UV irradiation and CPT treatment

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• 作者：Xiangzhong Zhao^a ; ^b ; Ning Wu^b ; Lili Ding^b ; Ming Liu^b ; Haizhou Liu^b ; Xiukun Lin^b ; ^c ; ^{linxiukun@yahoo.com}
• 关键词：p53 ; Zebrafish ; RNA binding protein ; Translational regulation
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：24 April, 2012
• 卷：586
• 期：8
• 页码：1220-1225
• 文件大小：602 K

摘要

p53 protein is an important regulatory factor involved in cell growth and development. In our previous study, we demonstrated that recombined zebrafish p53 protein could specifically bind to its own mRNA in vitro. To determine if a similar interaction exists in zebrafish and if this interaction affects zebrafish development, in the present study, we investigated the interaction of p53 protein and its mRNA in zebrafish embryos. Our results revealed that expressed zebrafish p53 protein could bind with its own mRNA in zebrafish embryos. Furthermore, the endogenous activated or ectopically expressed p53 protein could enhance the relative activity of Renilla luciferase fused with p53 3鈥睻TR in response to UV irradiation and CPT treatment, and retarded development of zebrafish embryos was observed.