miR-127-5p targets the 3鈥?/span>UTR of human 尾-F1-ATPase mRNA and inhibits its translation
- 作者：Imke M. Willers ; Inmaculada Martí ; nez-Reyes ; Marta Martí ; nez-Diez ; José ; M. Cuezva ; jmcuezva@cbm.uam.es
- 关键词：尾- 伪- and 纬-F1-ATPase ; subunits 尾 ; 伪 and 纬 of the mitochondrial H+-ATP synthase ; 尾- and 纬-mRNA ; mRNAs of subunits 尾- and 纬-F1-ATPase ; G3BP1 ; Ras-GAP SH3 binding protein 1 ; GFP ; green fluorescent protein ; SDH-B ; succinate dehydrogenase subunit B
- 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
- 出版年：2012
- 期刊代码：13_00052728
- 类别：bio
- 出版时间：May, 2012
- 卷：1817
- 期：5
- 页码：838-848
- 文件大小：1249 K
摘要
The mitochondrial H+-ATP synthase is a bottleneck component in the provision of metabolic energy by oxidative phosphorylation. The expression of its catalytic subunit (尾-F1-ATPase) is stringently controlled at post-transcriptional levels during oncogenesis, the cell cycle and in development. Here we show that miR-127-5p targets the 3鈥睻TR of 尾-F1-ATPase mRNA (尾-mRNA) significantly reducing its translational efficiency without affecting 尾-mRNA abundance. Despite the reduced expression of 尾-F1-ATPase in most human carcinomas, we observed no expression of miR-127-5p in different human cancer cell lines, minimizing the potential role of miR-127-5p as a regulator of the bioenergetic activity of mitochondria in cancer. In contrast, miR-127-5p is highly over-expressed in the human fetal liver. Consistent with previous findings in the rat, the expression of 尾-F1-ATPase in the human liver also seems to be controlled at post-transcriptional levels during development, what might suggest a role for miR-127-5p in controlling 尾-mRNA translation and thus in defining the bioenergetic activity of human liver mitochondria. Moreover, immunolocalization techniques and subcellular fractionation experiments using different antibodies against 尾-F1-ATPase reveal that the ectopic expression of 尾-F1-ATPase at the cell surface of the hepatocytes and HepG2 cells is negligible or stands for scrutiny.