- 作者：Carrie L. Geisbauer ; Ph.D.&lowast ; ; Benjamin M. Wu ; D.D.S. ; Ph.D.&lowast ; ; &dagger ; ; § ; ; James C.Y. Dunn ; M.D. ; Ph.D.&lowast ; ; &dagger ; ; &Dagger ; ; jdunn@mednet.ucla.edu
- 关键词：benzylalkonium chloride ; cell transplantation ; enteric nervous system ; Hirschsprung's disease ; neural crest stem cells
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：July, 2012
- 卷：176
- 期：1
- 页码：20-28
- 文件大小：1623 K
Background
Enteric cells, a mixture of cells isolated from the longitudinal and circular muscle of the gut, may contain neural crest stem cells (NCSCs) and therefore may be a potential source to regenerate the enteric nervous system.Materials and Methods
Benzylalkonium chloride (BAC) was employed to ablate the myenteric and submucosal plexi of the rodent jejunum. Enteric cells were then injected into this BAC-treated segment of the jejunum either with or without basic fibroblast growth factor (bFGF) mixed in collagen.
Results
Expression of peripherin, S100, and synaptophysin were found in all of the cell injection sites. Peripherin and S100 expression appeared in close proximity in ganglion-like structures when bFGF was injected simultaneously with enteric cells. Synapses that were formed in the presence of bFGF were elongated compared with those formed in the absence of exogenously delivered bFGF. A small percentage of enteric cells expressed peripherin in the injection site after transplantation.
Conclusions
Enteric cells transplanted with collagen and bFGF in an aganglionic segment of jejunum regenerated ganglion-like structures and may hold potential as a cellular therapeutic for various motility disorders of the gastrointestinal tract.