Neuronal nitric oxide synthase inhibition attenuates the development of L-DOPA-induced dyskinesia in hemi-Parkinsonian rats

详细信息	查看全文 | 推荐本文 |

• 作者：Kazuhiro Takuma^a ; Tatsunori Tanaka^a ; Tsuyoshi Takahashi^a ; Naoki Hiramatsu^a ; Yuki Ota^a ; Yukio Ago^a ; Toshio Matsuda^a ; ^b ; ^{matsuda@phs.osaka-u.ac.jp}
• 关键词：l-DOPA ; Hemi-Parkinsonian model ; Dyskinesia ; Nitric oxide ; Neuronal nitric oxide synthase
• 刊名：European Journal of Pharmacology
• 出版年：2012
• 期刊代码：24_00142999
• 类别：neu
• 出版时间：15 May, 2012
• 卷：683
• 期：1-3
• 页码：166-173
• 文件大小：643 K

摘要

Long-term treatment with the dopamine precursor levodopa (l-DOPA) frequently induces dyskinesia in Parkinson's disease patients, which is a major complication of this therapy. Previous studies using animal models show that repeated administration of l-DOPA results in alterations of some signaling molecules, including 螖FosB, phospho-DARPP32 and phosoho-GluA1 (also referred to as GluR1 or GluR-A) AMPA receptor subunits. Moreover, an in vivo microdialysis study showed that l-DOPA increases nitric oxide (NO) production in the striatum. However, it is not known whether NO is involved in the development of dyskinesia. The present study examined the effects of NOS inhibitors on the development of l-DOPA-induced dyskinesia in the rats. Dyskinesia symptoms were triggered by daily administration of l-DOPA for 3-4 weeks in unilateral 6-hydroxydopamine lesioned rats. Repeated treatments, 30 min prior l-DOPA administration, of the nonselective NOS inhibitor, N^G-nitro-l-arginine methyl ester, and the nNOS inhibitor 7-nitroindazole, but not the inducible NOS inhibitor aminoguanidine, attenuated the development of l-DOPA-induced dyskinesia. In agreement with the behavioral analysis, 7-nitroindazole reduced the l-DOPA-induced increases in 螖FosB, phospho-DARPP32 and phospho-GluA1 AMPA receptor subunit levels in the striatum of 6-hydroxydopamine-lesioned rats. Furthermore, aminoguanidine did not affect 螖FosB or phospho-GluA1 AMPA receptor subunit levels. These findings suggest that nNOS-derived NO is involved in the development of l-DOPA-induced dyskinesia through a post-synaptic mechanism.