Up-regulation of telomere-binding TRF1, TRF2 related to reactive oxygen species induced by As₂O₃ in MGC-803 cells

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• 作者：Zhang ; Yan ; Cao ; En-Hua ; Qin ; Jing-Fen
• 关键词：Apoptosis ; TRF1 ; TRF2 ; Reactive oxygen species ; Arsenic trioxide ; Telomeric state
• 刊名：European Journal of Pharmacology
• 出版年：2005
• 期刊代码：24_00142999
• 类别：neu
• 出版时间：May 23, 2005
• 卷：516
• 期：1
• 页码：1-9
• 文件大小：407 K

摘要

In this work, our study focused on As₂O₃ action in view point of telomere. Results showed that treatment of human gastric cancer MGC-803 cells with arsenic trioxide could cause up-regulation of telomeric repeat binding factor TRF1 and TRF2 mRNA and protein levels, and induced G2/M phase arrest and cell apoptosis. At the same time, telomere length shortening and telomerase inhibitory were not obvious. Flow cytometry measurements indicated that the increase of TRF1 and TRF2 proteins is related to oxidative stress by arsenic trioxide. Results also indicate that after arsenic trioxide treatment, p53 protein levels increased significantly and also could bind directly at the telomere t-loop junction. These findings demonstrate arsenic trioxide-induced cell cycle arrest and apoptosis might involve a novel pathway related to TRF1, TRF2 protein.