K, Ca complexes with a sulfonic ligand: Structure and DNA-binding properties

详细信息	查看全文 | 推荐本文 |

• 作者：Jiahe Luo^a ; Zhaorong Ma^b ; Huang Liang^b ; Jiwen Chen^a ; Zhengzhi Zeng^b ; ^{zengzhzh@yahoo.com.cn}
• 关键词：DMF ; N ; N-dimethyl formamide ; UV&ndash ; Vis ; UV&ndash ; visible ; CT-DNA ; calf thymus DNA ; Tris ; tris(hydroxymethyl) aminomethane ; TMS ; tetramethylsilane ; FAB ; fast-atom bombardment ; EB ; ethidium bromide
• 刊名：Spectrochimica Acta Part A ; Molecular and Biomolecular Spectroscopy
• 出版年：2012
• 期刊代码：99_13861425
• 类别：ch
• 出版时间：May, 2012
• 卷：90
• 期：Complete
• 页码：202-207
• 文件大小：591 K

摘要

A novel 4-(2,4-dihydroxybenzylideneamino)benzenesulfonic acid (HL), and its kalium(I), calcium(II) complexes [M(L)n]路2nH₂O路Cl_n (M = K(1) n = 1, M = Ca(2) n = 2), have been prepared and characterized. The crystal and molecular structures of 1 and 2 were determined by single-crystal X-ray diffraction. The interaction of 1, 2 and ligand (L) with calf thymus DNA was investigated by UV-visible (UV-vis), fluorescence and viscosity measurements. Experimental results indicate that 1, 2 and L could bind to DNA via the intercalation mode, and the binding affinity of 1 is stronger than that of 2 and L. The intrinsic binding constants of 1, 2 and L were 5.60 脳 10⁵, 6.53 脳 10⁵ and 1.44 脳 10⁵ M^鈭?, respectively. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The results indicated that 1 and 2 could cleave pBR322 DNA.