Determination of verapamil hydrochloride with 12-tungstophosphoric acid by resonance Rayleigh scattering method coupled to flow injection system
- 作者：Dongpo Xu ; Shaopu Liu ; Zhongfang Liu ; Xiaoli Hu
- 关键词：Resonance Reyleigh scattering ; 12-Tungstophosphoric acid ; Verapamil hydrochloride ; Flow injection analysis
- 刊名：Analytica Chimica Acta
- 出版年：2007
- 期刊代码：2_00032670
- 类别：ch
- 出版时间：4 April 2007
- 卷：588
- 期：1
- 页码：10-15
- 文件大小：164 K
摘要
A flow injection analysis (FIA) method coupled to resonance Rayleigh scattering (RRS) detection for the determination of verapamil hydrochloride (VP) was proposed. In pH 1.0 acidic medium, 12-tungstophosphoric acid (TP) reacted with VP to form an ion-association complex, which resulted in a significant enhancement of RRS intensity. The maximum scattering peak was located at 293 nm. RRS intensity was proportional to the concentration of VP in the range of 0.017–13.0 μg mL"12;1, and the detection limit (3σ) was 5.1 ng mL"12;1. The proposed method exhibited satisfactory reproducibility with a relative standard derivation (R.S.D.) of 2.1%for 11 successive determinations of 5.0 μg mL"12;1 VP. Therefore, a novel method for the determination of VP by FIA–RRS was developed. The optimum reaction conditions and the parameters of the FIA operation such as flow rate, injection volume, reactor length, and so on had been optimized in this paper. The present method had been applied to the determination of VP in serum samples and pharmaceuticals with satisfactory results. The maximal sample throughput in the optimized system was 80 h"12;1.