Identification and quantification method of spiramycin and tylosin in feedingstuffs with HPLC–UV/DAD at 1ppm level

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• 作者：Civitareale ; C. ; Fiori ; M. ; Ballerini ; A. ; Brambilla ; G.
• 关键词：Growth promoters ; Animal feed additives ; Tylosin ; Spiramycin ; Illegal use ; HPLC
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2004
• 期刊代码：65_07317085
• 类别：ch
• 出版时间：October 29, 2004
• 卷：36
• 期：2
• 页码：317-325
• 文件大小：347 K

摘要

The use of the two macrolides antibiotics Spiramycin (S) and Tylosin (T) as growth promoters in animal feeding has been recently withdrawn in the European Union due to a concern about the outbreaks of farmacoresistance fenomena as a possible hazard for humans. For feed additives monitoring purposes, an analytical method has been developed for their extraction, purification and identification in different animal feedingstuffs (pelleted beef, pig, poultry feeds and calves milk replacer) at a minimum performance required limit (MRPL) of 1μgg⁻¹ (ppm). Such limit has been established according to the lowest dosage of additives still able to elicit an appreciable growth promoting effect.