H1.X with different properties from other linker histones is required for mitotic progression
- 作者：Hideaki Takata ; Sachihiro Matsunaga ; Akihiro Morimoto ; Rika Ono-Maniwa ; Susumu Uchiyama ; Kiichi Fukui
- 关键词：Histone H1 ; Histone H1.X ; Chromosome ; RNAi ; FRAP ; Mitosis
- 刊名：FEBS Letters
- 出版年：2007
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：7 August 2007
- 卷：581
- 期：20
- 页码：3783-3788
- 文件大小：635 K
摘要
We report here the characterization of H1.X, a human histone H1 subtype. We demonstrate that H1.X accumulates in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. In addition, the results of fluorescence recovery after photobleaching indicate that the exchange of H1.X on and off chromatin is faster than that of the other H1 subtypes. Furthermore, RNA interference experiments reveal that H1.X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes.