High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates

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• 作者：Toshimi Hasegawa¹ ; Manabu Hayashi¹ ; Kazuki Nomura¹ ; Mayumi Hayashi¹ ; Miyuki Kido² ; Tsuneo Ohmori² ; Masao Fukuda³ ; Akinori Iguchi¹ ; Shigeaki Ueno⁴ ; Toru Shigematsu¹ ; ^{shige@nupals.ac.jp} ; Masao Hirayama¹ ; ⁵ ; Tomoyuki Fujii¹ ; ⁴
• 关键词：Microplate ; High-throughput ; High-pressure ; Microbial inactivation ; Escherichia coli ; Saccharomyces cerevisiae
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2012
• 期刊代码：pc9_13891723
• 类别：ce
• 出版时间：June, 2012
• 卷：113
• 期：6
• 页码：788-791
• 文件大小：559 K

摘要

Using microplates as pressure and cultivation vessels, a high-throughput method was developed for analyzing the high-pressure inactivation kinetics of microorganisms. The loss of viability from a high-pressure treatment, measured based on the growth delay during microplate cultivation, showed reproducibility with the conventional agar plate method and was applicable for the kinetics analysis.