Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

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• 作者：M.S. Benhabiles^a ; N. Abdi^a ; N. Drouiche^a ; ^c ; ^{nadjibdrouiche@yahoo.fr} ; H. Lounici^a ; A. Pauss^b ; M.F.A. Goosen^d ; N. Mameri^b
• 关键词：Enzyme ; Protein hydrolysate ; Ultrafiltration ; Bioreactor ; Biochemical engineering ; Food processing
• 刊名：Materials Science and Engineering ; C
• 出版年：2012
• 期刊代码：63_09284931
• 类别：ms
• 出版时间：1 May, 2012
• 卷：32
• 期：4
• 页码：922-928
• 文件大小：626 K

摘要

The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH = 1.5-2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 掳C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m^鈭?#xA0;2 bar^鈭?#xA0;1. However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs.