Adsorption kinetics of plasma proteins on ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles

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• 作者：M. Jansch^a ; ^{mirko.jansch@gmx.de} ; P. Stumpf^b ; ^{patstu@chemie.fu-berlin.de} ; C. Graf^b ; ^{cmgraf@zedat.fu-berlin.de} ; E. Rü ; hl^b ; ^{ruehl@zedat.fu-berlin.de} ; R.H. Mü ; ller^a ; ^{nanoteam@gmx.com}
• 关键词：Protein adsorption ; 2-D PAGE ; Drug targeting ; Biodistribution ; USPIO ; Iron oxide nanoparticles
• 刊名：International Journal of Pharmaceutics
• 出版年：2012
• 期刊代码：24_03785173
• 类别：pha
• 出版时间：30 May, 2012
• 卷：428
• 期：1-2
• 页码：125-133
• 文件大小：755 K

摘要

In this study the kinetics of plasma protein adsorption onto ultrasmall superparamagnetic iron oxide (USPIO) particles have been analyzed and compared to previously published kinetic studies on polystyrene particles (PS particles), oil-in-water nanoemulsions and solid lipid nanoparticles (SLNs). SPIO and USPIO nanoparticles are commonly used as magnetic resonance imaging (MRI) enhancers for tumor imaging as well as in drug delivery applications. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) has been used to determine the plasma protein adsorption onto the citrate/triethylene glycol-stabilized iron oxide surface. The results indicate that the existence of a Vroman effect, a displacement of previously adsorbed abundant proteins, such as albumin or fibrinogen, respectively, on USPIO particles has to be denied. Previously, identical findings have been reported for oil-in-water nanoemulsions. Furthermore, the protein adsorption kinetics differs dramatically from that of other solid drug delivery systems (PS, SLN). More relevant for the in vivo fate of long circulating particles is the protein corona after several minutes or even hours. Interestingly, the patterns received after an incubation time of 0.5 min to 240 min are found to be qualitatively and quantitatively similar. This leads to the assumption of a long-lived (鈥渉ard鈥? protein corona around the iron oxide nanoparticles.