Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

详细信息	查看全文 | 推荐本文 |

• 作者：Myung-Ik Yoo^a ; ^b ; Yeong-Ju Seo^c ; Kyu-Sil Choi^c ; Jeong Sook Ha^b ; Kyoungja Woo^a ; ^{kjwoo@kist.re.kr}
• 关键词：Superparamagnetic iron oxide nanoparticles (SPIONs) ; Scatteredly cRGDfK-conjugated ; Ultrasmall ; Water-dispersible ; Lipophilic ; Cellular uptake
• 刊名：Colloids and Surfaces B ; Biointerfaces
• 出版年：2012
• 期刊代码：14_09277765
• 类别：ms
• 出版时间：1 September, 2012
• 卷：97
• 期：Complete
• 页码：175-181
• 文件大小：1063 K

摘要

We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin 伪_v尾₃) and MCF-7 cells (human breast cancer cells, express low level of integrin 伪_v尾₃) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin 伪_v尾₃-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.