Morphology and ultrastructure of the bacterial receptacle in Steinernema nematodes (Nematoda: Steinernematidae)

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• 作者：Sam Kyu Kim¹ ; Yolanda Flores-Lara² ; S. Patricia Stock ; ^{spstock@email.arizona.edu}
• 关键词：Steinernema ; Xenorhabdus ; Bacterial receptacle ; Morphology ; Ultrastructure ; TEM ; DIC microscopy ; Interspecific variation
• 刊名：Journal of Invertebrate Pathology
• 出版年：2012
• 期刊代码：120_00222011
• 类别：abs
• 出版时间：July, 2012
• 卷：110
• 期：3
• 页码：366-374
• 文件大小：2203 K

摘要

Infective juveniles of entomopathogenic nematodes in the genus Steinernema harbor symbiotic bacteria, Xenorhabdus spp., in a discrete structure located in the anterior portion of the intestine known as the 鈥榖acterial receptacle鈥?(formerly known as the bacterial or intestinal vesicle). The receptacle itself is a structured environment in which the bacteria are spatially restricted. Inside this receptacle, bacterial symbionts are protected from the environment and grow to fill the receptacle. Until now, no comparative study across different Steinernema spp. has been undertaken to investigate if morphological variation in this structure exists at the interspecific level. In this study, we examined the bacterial receptacles of 25 Steinernema spp. representatives of the currently accepted five evolutionary clades. Our observations confirmed the bacterial receptacle is a modification of the two most anterior cells of the ventricular portion of the intestine. Size of the bacterial receptacle varied across the examined species. Steinernema monticolum (clade II) had the largest receptacle of all examined species (average: 46 脳 17 渭m) and S. rarum (no clade affiliation) was noted as the species with the smallest observed receptacle (average: 8 脳 5 渭m). At the morphological level, species can be grouped into two categories based on the presence or absence of vesicle within the receptacle. The receptacles of all examined species harbored an intravesicular structure (IVS) with variable morphology. All examined taxa members of the 鈥榝eltiae鈥?(clade III) and 鈥榠ntermedium鈥?(clade II) clades were characterized by having a vesicle. This structure was also observed in S. diaprepesi (clade V), S. riobrave (clade IV) and S. monticolum (clade I).