Polymerase incorporation of pyrene-nucleoside triphosphates

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• 作者：Marcel Hollenstein ; ^{hollenstein@dcb.unibe.ch} ; Filip Wojciechowski ; Christian J. Leumann
• 关键词：Nucleosides ; DNA ; Terminal deoxynucleotidyl transferase (TdT) ; Nucleotides ; Triphosphate analogues
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 July, 2012
• 卷：22
• 期：13
• 页码：4428-4430
• 文件大小：458 K

摘要

Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.