Tumor necrosis factor 伪 (TNF伪) G-308A (rs 1800629), interleukin-6 (IL-6) G-174C (rs 1800795) and IL-10 G-1082A (rs 1800896) single nucleotide polymorphisms (SNPs) in DNA from peripheral blood leukocytes of 190 patients with HT and 231 healthy controls were investigated by real-time PCR combined with melting curve analysis using fluorescence-labeled hybridization probes.
There was no notable risk for HT afflicted by TNF伪 鈭?#xA0;308, IL-6 鈭?#xA0;174 and IL-10 鈭?#xA0;1082 polymorphisms alone. However, carriers of variant alleles of both IL-10 鈭?#xA0;1082 and TNF伪 鈭?#xA0;308 polymorphisms had four-fold times higher risk for HT in comparison with non-carriers. Additionally, concomitant presence of both mutant IL-10 鈭?#xA0;1082 A and IL-6 鈭?#xA0;174 C alleles raised three-fold the HT risk.
Our results suggest that the combined effects of TNF伪 鈭?#xA0;308, IL-6 鈭?#xA0;174 and IL-10 鈭?#xA0;1082 variant alleles may be more decisive to induce functional differences and modify the risk for HT.