Validation of an analytical method to determine sulfamides in kidney by HPLC-DAD and PARAFAC2 with first-order derivative chromatograms

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• 作者：I. Garcí ; a ; M.C. Ortiz ; L. Sarabia ; J.M. Aldama
• 关键词：PARAFAC2 ; Sulfonamides ; First-order derivative ; Three-way models ; Baseline drift ; Decision limit CCα ; Capability of detection CCβ ; Decision 2002/657/EC
• 刊名：Analytica Chimica Acta
• 出版年：2007
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：28 March 2007
• 卷：587
• 期：2
• 页码：222-234
• 文件大小：682 K

摘要

Six sulfamides were extracted from kidney and analysed by high-performance liquid chromatography with diode array detection (HPLC-DAD): sulfadiazine, sulfamethazine, sulfamethoxypyridazine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline.

Two main difficulties arose in identifying and quantifying the analytes. Firstly, the chromatographic peaks of the matrix interferences overlapped with those of the analytes. The uniqueness property of PARAFAC2 solved this problem. Secondly, the gradient elution caused a baseline drift. The first-order derivative of the chromatograms minimized its effect.

The analytical method was validated. As the performance criteria detailed in the European Decision 2002/657/EC are based on specific signals, this paper generalizes those criteria for higher-order and non-specific signals. In this sense the proposed methodology is general and can be applied to any chromatographic method (HPLC or GC) with a detector that provide a multivariate signal (MS, DAD, EC, etc.).