Malachite green residues in farmed fish in Croatia

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• 作者：Nina Biland啪i膰^a ; ^{bilandzic@veinst.hr} ; Ivana Varenina^a ; Bo啪ica Solomun Kolanovi膰^a ; Dra啪en Orai膰^b ; Snje啪ana Zrn膷i膰^b
• 关键词：Malachite green ; Carp ; Trout ; Enzyme-immunoassay
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：August, 2012
• 卷：26
• 期：2
• 页码：393-396
• 文件大小：141 K

摘要

Malachite green concentrations were determined in muscle tissues samples of 42 carp (Cyprinus carpio) and 30 rainbow trout (Oncorhynchus mykiss) collected from fish farms in Croatia from 2009 to 2011. Concentrations were determined using the immunoassay method, validated according to the criteria of Commission Decision 2002/657/EC. The method performance is appropriate for the detection of malachite green, due to the obtained CC尾 (0.68聽渭g聽kg^鈭?) and LOD (0.31聽渭g聽kg^鈭?) values that were below the MRPL level of 2聽渭g聽kg^鈭?. Malachite green levels above the limit of detection of the method were determined in 18.1%of samples tested. The mean malachite green level (0.231聽渭g聽kg^鈭?) of samples tested was lower than the determined limit of detection of the method. Concentrations ranged from 0.1 to 0.3聽渭g聽kg^鈭? in 68.1%of all fish samples. The measured concentrations exceeded 1聽渭g聽kg^鈭? in only two samples and the highest concentration (1.07聽渭g聽kg^鈭?) was measured in rainbow trout muscle. Since this study is limited to malachite green residues, further investigation is required for determination of its metabolite, leucomalachite green. Additional surveillance programmes with a larger number of fish muscle samples should be carried out to improve the risk assessment.