Polyelectrolyte LbL microcapsules versus PLGA microparticles for immunization with a protein antigen

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• 作者：Marie-Luce De Temmerman^a ; Joanna Rejman^a ; Roosmarijn E. Vandenbroucke^b ; ^c ; Stefaan De Koker^d ; Claude Libert^b ; ^c ; Johan Grooten^d ; Jo Demeester^a ; Bruno Gander^e ; Stefaan C. De Smedt^a ; ^{ilse.dupon@ugent.be}
• 关键词：PLGA ; Microcapsules ; Layer-by-Layer ; Cytokines ; Antigen delivery ; Microparticles
• 刊名：Journal of Controlled Release
• 出版年：2012
• 期刊代码：25_01683659
• 类别：pha
• 出版时间：10 March, 2012
• 卷：158
• 期：2
• 页码：233-239
• 文件大小：499 K

摘要

The transition from organism-based traditional vaccines to the use of safer subunit vaccines has implemented the use of adjuvants to enhance immunogenicity. This study compares the potential of two types of polymeric microparticles as delivery systems for the model antigen ovalbumin. The delivery systems encompassed polyelectrolyte microcapsules, assembled via Layer-by-Layer technology, and PLGA microparticles fabricated by spray-drying. Mice were immunized subcutaneously either by a single injection or by two injections separated by four weeks with an equivalent dose of the OVA-loaded particles. Both particulate formulations mediated high, long-term IgG₁ responses whereas the IgG_2c titers remained low. Additionally, Th1 and Th2 phenotype immune responses against OVA were assessed by quantifying the production of cytokines in CD4 + T-cells derived from the spleens of immunized mice at 6 months after the first injection. Immunization with particulate formulations led to significantly increased IL-2, IL-4, IL-10 and IFN-纬 production by splenic CD4 + T-cells compared to control animals. LbL microcapsules and PLGA microparticles generated strong immune responses in vivo, characterized by a mixed Th1/Th2 type response with predominance of Th2 immunity. Both particulate formulations elicited a comparable type of immune response and appear to be promising for antigen delivery.