IL-7 splicing variant IL-7未5 induces human breast cancer cell proliferation via activation of PI3K/Akt pathway

详细信息	查看全文 | 推荐本文 |

• 作者：Deshun Pan^a ; ^b ; ¹ ; Bing Liu^b ; ¹ ; Xiaobao Jin^a ; ¹ ; Jiayong Zhu^a ; ^{zhujiayong888@163.com}
• 关键词：IL-7 variant ; IL-7未5 ; Human breast cancer ; Proliferation ; Cell cycle ; Cyclin D1 ; p27kip1 ; PI3K/Akt
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：15 June, 2012
• 卷：422
• 期：4
• 页码：727-731
• 文件大小：418 K

摘要

Various tumor cells express interleukin 7 (IL-7) and IL-7 variants. IL-7 has been confirmed to stimulate solid tumor cell proliferation. However, the effect of IL-7 variants on tumor cell proliferation remains unclear. In this study, we evaluated the role of IL-7未5 (an IL-7 variant lacking exon 5) on proliferation and cell cycle progression of human MDA-MB-231 and MCF-7 breast cancer cells. The results showed that IL-7未5 promoted cell proliferation and cell cycle progression from G1 phase to G2/M phase, associated with upregulation of cyclin D1 expression and the downregulation of p27^kip1 expression. Mechanistically, we found that IL-7未5 induced the activation of Akt. Inhibition of PI3K/Akt pathway by LY294002 reversed the proliferation and cell cycle progression of MDA-MB-231 and MCF-7 cells induced by IL-7未5. In conclusion, our findings demonstrate that IL-7未5 variant induces human breast cancer cell proliferation and cell cycle progression via activation of PI3K/Akt pathway. Thus, IL-7未5 may be a potential target for human breast cancer therapeutics intervention.