A great number of physiological processes are regulated by the release of ectodomains of membrane proteins. A Disintegrin And Metalloprotease 1
7 (ADAM1
7) is one of the important enzymes, which mediate this process called shedding. Today, more than
70 substrates of this transmembrane metalloprotease are known. This broad spectrum raises the question how ADAM1
7 recognizes its substrates specifically. Differently tagged ADAM1
7 deletion
variants were used to demonstrate that exclusively the extracellular domains of ADAM1
7 are needed for interaction with two of its substrates, the
IL-6R and the IL-1RII; whereas the transmembrane- and cytoplasmic-region are dispensable for this process. In the extracellular part solely the membrane-proximal domain of ADAM1
7 is mandatory for recognition of the two type-I transmembrane proteins, but not for the interaction with the type-II transmembrane molecule TNF-伪.
Structured summary of protein interactions
with by () with by ()