Hemoglobin adducts as a measure of variations in exposure to acrylamide in food and comparison to questionnaire data

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• 作者：Anna C. Vikströ ; m^a ; Margareta Warholm^b ; Birgit Paulsson^a ; Anna Axmon^c ; Elisabet Wirfä ; lt^d ; Margareta Tö ; rnqvist^a ; ^{margareta.tornqvist@mmk.su.se}
• 关键词：Acrylamide ; Dietary intake ; Glycidamide ; Dietary history methodology ; Individual variation ; Food frequency questionnaire
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：July, 2012
• 卷：50
• 期：7
• 页码：2531-2539
• 文件大小：644 K

摘要

Measurement of haemoglobin (Hb) adducts from acrylamide (AA) and its metabolite glycidamide (GA) is a possibility to improve the exposure assessment in epidemiological studies of AA intake from food. This study aims to clarify the reliability of Hb-adduct measurement from individual single samples for exposure assessment of dietary AA intake.

The intra-individual variations of AA- and GA-adduct levels measured in blood samples collected over 20 months from 13 non-smokers were up to 2-fold and 4-fold, respectively. The corresponding interindividual variations observed between 68 non-smokers, with large differences in AA intake, were 6-fold and 8-fold, respectively. The intra-individual variation of the GA-to-AA-adduct level ratio was up to 3-fold, compared to 11-fold between individuals (n = 68).

From AA-adduct levels the average AA daily intake (n = 68) was calculated and compared to that estimated from dietary history methodology: 0.52 and 0.67 渭g/kg body weight and day, respectively. At an individual level the measures showed low association (Rs = 0.39).

Conclusions

Dietary AA is the dominating source to measured AA-adduct levels and corresponding inter- and intra-individual variations in non-smokers. Measurements from single individual samples are useful for calculation of average AA intake and its variation in a cohort, and for identification of individuals only from extreme intake groups.