Cholesterol-to-phospholipid ratio in whole sperm and seminal plasma from fertile stallions and stallions with unexplained subfertility
- 作者：S.P. Brinsko ; C.C. Love ; J.E. Bauer ; M.L. Macpherson ; D.D. Varner
- 关键词：Stallion ; Semen ; Cholesterol ; Phospholipid ; Subfertility
- 刊名：Animal Reproduction Science
- 出版年：2007
- 期刊代码：8_03784320
- 类别：abs
- 出版时间：May 2007
- 卷：99
- 期：1-2
- 页码：65-71
- 文件大小：175 K
摘要
Semen samples were collected from six fertile stallions and seven stallions with unexplained infertility. Percentages of motile sperm (77.5 ± 11.3 versus 67.5 ± 12.2, P = 0.2), and progressively motile sperm (70.8 ± 13.6 versus 60.7 ± 14.0, P = 0.2) were similar between fertile and subfertile stallions, respectively. Morphologic characteristics in ejaculates of control and affected stallions (%normal: 60.2 ± 18.2 versus 52.9 ± 11.3, P = 0.4;%abnormal heads 7.3 ± 4.8 versus 12.1 ± 5.0, P = 0.11; and%abnormal acrosomes 1.6 ± 2.1 versus 3.0 ± 3.4, P = 0.4) did not differ. After incubation with the calcium ionophore A23187, acrosome reaction rate of sperm from fertile stallions was 96 ± 2.8%whereas only 2.9 ± 2.5%of sperm from stallions with unexplained subfertility had acrosome reacted (P < 0.001). Molar amounts of cholesterol and phospholipid in whole sperm and seminal plasma did not differ (P > 0.1) between fertile and subfertile stallions. However, the molar ratio of cholesterol-to-phospholipid was 2.5 times greater in the seminal plasma (P = 0.09) and 1.9 times greater (P = 0.009) in whole sperm of subfertile stallions compared to fertile stallions.