Effect of daily semen centrifugation and resuspension on the longevity of equine sperm quality following cooled storage
- 作者：C.C. Love ; clove@cvm.tamu.edu ; T.L. Blanchard ; D.D. Varner ; S.P. Brinsko ; J. Voge ; S. Bliss ; K. Sudderth ; S. Teague ; K. LaCaze
- 关键词：Sperm ; Semen ; Storage ; Equine ; Horse
- 刊名：Theriogenology
- 出版年：2012
- 期刊代码：98_0093691x
- 类别：abs
- 出版时间：June, 2012
- 卷：77
- 期：9
- 页码：1911-1917
- 文件大小：429 K
摘要
An experiment was conducted to determine whether cooled semen quality could be maintained for a longer interval by conducting daily centrifugation of extended semen, with resuspension of the sperm pellet in fresh extender. Semen treatments included SP10NC and SP50NC which contained 10 and 50%seminal plasma, respectively, were not centrifuged (NC), and were stored at 4 to 7 掳C for 96 h. Treatments SP10C and SP50C contained 10 and 50%seminal plasma, respectively, but were centrifuged (C) after 24, 48, and 72 h of cooled storage, with daily resuspension in fresh extender containing 10%seminal plasma. Percent total sperm motility (TMOT) and progressively motile (PMOT) was reduced (P < 0.05) in the SP50NC treatment after 24, 48, 72, and 96 h of storage, and TMOT did not differ (P > 0.05) in the SP10C, SP50C, SP10NC groups after the same storage periods. The%COMP-伪t did not differ (P > 0.05) among treatments at any time period. Percent membrane intact sperm (SMI) was reduced in SP50NC, as compared to SP10C at 48, 72, and 96 h (P < 0.05). Daily centrifugation and resuspension of sperm exposed to 50%seminal plasma for the first 24 h (SP50C) yielded similar TMOT, PMOT, VCL, SMI,%COMP-伪t (P > 0.05) to Groups SP10NC and SP10C after 96 h of storage. Daily centrifugation and resuspension of cool-stored equine semen in fresh extender may be a method to increase sperm longevity.