Limited synergistic effect of antisense oligonucleotides against bcr-abl and transferrin receptor mRNA in leukemic cells in culture
- 作者：Vasconcelos ; M. Helena ; Beleza ; Sandra S. ; Quirk ; Catriona ; Maia ; Luí ; s F. ; Sambade ; Clara ; et. al.
- 关键词：Antisense oligonucleotide ; Leukemia ; bcr-abl ; Transferrin receptor
- 刊名：Cancer Letters
- 出版年：2000
- 期刊代码：44_03043835
- 类别：med
- 出版时间：May 1, 2000
- 卷：152
- 期：2
- 页码：135-143
- 文件大小：262 K
摘要
The synergistic use of antisense oligonucleotides (ASOs) towards the bcr-abl and the transferrin receptor (TfR) mRNA was studied in a chronic myeloid leukemia (CML) cell line, aiming to improve the efficiency of individual ASO treatment. At 20 μM concentration, bcr-abl ASOs reduced cell growth by 40%and was specific for cells that have the translocation: there was a 34%reduction of BCR-ABL protein. The TfR ASO reduced cell growth by 20%and decreased TfR protein by 24%. The ASOs were more potent at reducing cell growth when used in combination (respectively, −20 and −17%than bcr-abl ASO and TfR ASO when used individually at the 10 μM concentration), thus we postulate that there is synergism of action. Cell cycle analysis also revealed that the sub-G1 peak was bigger in the synergistic treatment.