In vivo subcellular localization of Mal de R铆o Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
- 作者：Guillermo A. Maroniche ; Vanesa C. Mongelli ; Gabriela Llauger ; Victoria Alfonso ; Oscar Taboga ; Mariana del Vas ; mdelvas@cnia.inta.gov.ar
- 关键词：MRCV ; Fijivirus ; Subcellular localization ; Non-structural proteins ; GFP ; Confocal microscopy
- 刊名：Virology
- 出版年：2012
- 期刊代码：108_00426822
- 类别：imm
- 出版时间：1 September, 2012
- 卷：430
- 期：2
- 页码：81-89
- 文件大小：1265 K
摘要
The in vivo subcellular localization of Mal de R铆o Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and 伪-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread.