The effect of pre-existing immunity on the capacity of influenza virosomes to induce cytotoxic T lymphocyte activity
- 作者：Arjan de Mare ; Laura B. Bungener ; Joke Regts ; Jacqueline de Vries-Idema ; Ate G.J. van der Zee ; Jan Wilschut ; Toos Daemen
- 关键词：Virosome ; CTL ; Pre-existing immunity
- 刊名：Vaccine
- 出版年：2008
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：2 May 2008
- 卷：26
- 期：19
- 页码：2314-2321
- 文件大小：393 K
摘要
Protein antigens encapsulated in virosomes generated from influenza virus can induce antigen-specific cytotoxic T lymphocyte (CTL) responses. In the present study we determined, in a murine model system, whether pre-existing immunity against influenza virus hampers the induction of a CTL response. CTL induction was only slightly reduced by pre-injection of influenza virus-specific antibodies or pre-exposure to influenza virus. Both pretreatments resulted in the same level of reduction, suggesting that virus-specific antibodies rather than T cell responses account for the reduction. Furthermore, a booster immunization enhanced CTL activation, indicating that virosome-specific immunity induced by a prime immunization does not hamper the booster effect. In conclusion, CTL induction against virosome-encapsulated protein antigens is not significantly inhibited by pre-existing humoral or cellular immunity against influenza virus.