Anthocyanin identification and composition of wild Vitis spp. accessions by using LC-MS and LC-NMR

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• 作者：Alexander Acevedo De la Cruz^a ; Ghislaine Hilbert^b ; Cé ; line Riviè ; re^a ; Virginie Mengin^a ; Nathalie Ollat^b ; Louis Bordenave^b ; Sté ; phane Decroocq^b ; Jean-Claude Delaunay^a ; Serge Delrot^c ; Jean-Michel Mé ; rillon^a ; Jean-Pierre Monti^a ; Eric Gomè ; s^c ; ^{eric.gomes@bordeaux.inra.fr} ; Tristan Richard^a ; ^{tristan.richard@u-bordeaux2.fr}
• 关键词：Anthocyanin ; Vitis spp. ; Grapevine ; LC&ndash ; MS ; LC&ndash ; NMR
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：30 June, 2012
• 卷：732
• 期：Complete
• 页码：145-152
• 文件大小：315 K

摘要

The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatography coupled to mass spectrometry (LC-MS) and NMR spectroscopy (LC-NMR) were used to separate and identify the structure of anthocyanins present in these species. Combination of LC-MS and LC-NMR data resulted in the identification of 33 anthocyanins. In particular, newly reported cis isomers of p-coumaric-derivatives were identified (petunidin-, peonidin- and malvidin-3-(6-p-coumaroyl)-5-diglucoside). In V. cinerea and V. vinifera, anthocyanins were monoglucoside derivatives whereas in V. amurensis and V. X champinii, both mono- and diglucoside derivatives were identified. Malvidin-, delphinidin- and petunidin-derivatives were, respectively, the most abundant components in V. cinerea and V. vinifera, V. amurensis and V. X champinii.