The hairy and enhancer of split 1 is a negative regulator of the repressor element silencer transcription factor
- 作者：Amar Abderrahmani ; Guy Niederhauser ; Vincent Lenain ; Romano Regazzi and Gé ; rard Waeber
- 关键词：Hairy and enhancer of split-1 ; Neuronal cells ; Beta cells ; REST ; Transcriptional repression
- 刊名：FEBS Letters
- 出版年：2005
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：2005
- 卷：579
- 期：27
- 页码：6199-6204
- 文件大小：288 K
摘要
Silencing of the transcriptional repressor REST is required for terminal differentiation of neuronal and β-cells. In this study, we hypothesized that REST expression is controlled by hairy and enhancer of split 1 (HES-1), a transcriptional repressor that plays an important role in brain and pancreas development. We identified several N elements (CTNGTG) within the promoter of REST and confirmed that HES-1 associates with the endogenous promoter of REST. Moreover, using a cells model that overexpress HES-1 and a combination of experimental approaches, we demonstrated that HES-1 reduces endogenous REST expression. Taken together, these results indicate that HES-1 is an upstream negative regulator of REST expression.