The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain
- 作者：Maria Eriksson ; Helena Samuelsson ; Eva-Britt Samuelsson ; Leyuan Liu ; Wallace L McKeehan ; Eirikur Benedikz ; Erik Sundströ ; m
- 关键词：N-Methyl-d-aspartate receptor ; NR3A ; Microtubule associated-protein ; MAP1S ; C19ORF5
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：14 September 2007
- 卷：361
- 期：1
- 页码：127-132
- 文件大小：493 K
摘要
When screening a brain cDNA library, we found that the N-methyl-d-aspartate receptor subunit NR3A binds to microtubule-associated protein (MAP) 1S/chromosome 19 open reading frame 5 (C19ORF5). The interaction was confirmed in vitro and in vivo, and binding of MAP1S was localized to the membrane-proximal part of the NR3A C-terminus. MAP1S belongs to the same family as MAP1A and MAP1B, and was found to be abundant in both postnatal and adult rat brain. In hippocampal neurons the distribution-pattern of MAP1S resembled that of β-tubulin III, but a fraction of the protein colocalized with synaptic markers synapsin and postsynaptic density protein 95 (PSD95), in β-tubulin III-negative filopodia-like protrusions. There was coexistance between MAP1S and NR3A immunoreactivity in neurite shafts and occasionally in filopodia-like processes. MAP1S potentially links NR3A to the cytoskeleton, and may stabilize NR3A-containing receptors at the synapse and regulate their movement between synaptic and extrasynaptic sites.