The virtual element in proximal promoter of porcine myostatin is regulated by myocyte enhancer factor 2C
- 作者：Jia Li
- 关键词：FOXO ; forkhead box protein O1 ; EMSA ; electrophoretic mobility gel shift assay ; MEF2 ; myocyte enhancer factor-2 ; siRNA ; small interfering RNA
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：9 March, 2012
- 卷：419
- 期：2
- 页码：175-181
- 文件大小：797 K
摘要
Myostatin (myostatin) is a member of the transforming growth factor-尾 superfamily. In this study, we investigated the transcriptional regulation of porcine myostatin expression in C2C12 cells. Sequence analysis of 2 kb of the porcine myostatin gene upstream region revealed that it contains 16 E-box motifs. Using a serial deletion strategy, we identified a vital enhancer region between nucleotides 鈭?18 and 鈭?37 in promoter region of porcine myostatin. Gel-shift assay and deletion analysis result showed F1 site, from nucleotides 鈭?06 to 鈭?89, was the key element to produce the promoter/nucleus extract complex, which directly interact with transcriptional factor MEF2C. Overexpression of MEF2C increased myostatin promoter activity in dose dependent manner; and whereas introducing of small interfering RNA to MEF2C produced the opposite effect in both myoblasts and myotubes. When endogenous MEF2C was knocked down, the complex of protein/F1 was attenuated, and myostatin mRNA and protein level was also decreased. Thus, we propose that MEF2C could modulate and restrain myogenesis by myostatin activation and myostatin-dependent gene processing in porcine.