Hippocampal NMDA receptor subunit expression and watermaze learning in estrogen deficient female mice

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• 作者：Wah Chin Boon ; Jeroen Diepstraten ; Jorien van der Burg ; Margaret E.E. Jones ; Evan R. Simpson and Maarten van den Buuse
• 关键词：Aromatase knockout ; ArKO ; Female ; Estrogen ; Hippocampus ; Long-term spatial memory ; N-methyl-d-aspartate (NMDA) receptor subunit ; Morris watermaze
• 刊名：Molecular Brain Research
• 出版年：2005
• 期刊代码：40_0169328x
• 类别：neu
• 出版时间：2005
• 卷：140
• 期：1-2
• 页码：127-132
• 文件大小：176 K

摘要

The aromatase knockout (ArKO) mouse is estrogen deficient. Using reverse-transcription and real-time PCR, we showed that transcript levels of the N-methyl-d-aspartate (NMDA) receptor subunit NR2B are significantly higher in the hippocampus of female ArKO mice compared to wild-type (WT) littermates. Expression levels of NR1, NR2A, but not NR2C, also tended to be higher in ArKO mice. In the Morris watermaze test for spatial memory, both genotypes displayed equal significant improvement in the latency in locating the invisible platform over the 5-day training period. These findings show that selective loss of estrogen synthesis is associated with changes in NMDA receptor subunit expression in the hippocampus but little change in spatial learning ability.