Polymorphisms of the insulin-like growth factor-binding protein 3 gene (IGFBP3) in gayal (Bos frontalis)
- 作者：Dongmei Xi ; Min Wu ; Yueyuan Fan ; Qing Liu ; Jing Leng ; Xiao Gou ; Huaming Mao ; Weidong Deng ; dwdxdm@yahoo.com.cn
- 关键词：He ; gene heterozygosity ; Ho ; gene homozygosity ; H&ndash ; W ; Hardy&ndash ; Weinberg ; IGFBP3 ; insulin-like growth factor-binding protein 3 gene ; Ne ; effective allele numbers ; PCR ; polymerase chain reaction ; PIC ; polymorphism information content ; RFLP ; restrict
- 刊名：Gene
- 出版年：2012
- 期刊代码：73_03781119
- 类别：bio
- 出版时间：10 April, 2012
- 卷：497
- 期：1
- 页码：98-102
- 文件大小：867 K
摘要
The gene coding for insulin-like growth factor-binding protein 3 (IGFBP3) is important for regulation of growth, development and metabolism in mammals. The present investigation was conducted to study nucleotide polymorphism of the IGFBP3 in gayal (Bos frontalis) and to compare the variations with those which occur in other ruminants. A fragment of 645 base pairs of the IGFBP3 covering a part of exon 2, the complete intron 2 and exon 3 and a part of intron 3 was amplified, sequenced (n = 46) and digested (n = 79) with HaeIII restriction enzyme from 125 collected gayal samples. Nine single nucleotide polymorphisms (SNPs) [C14T, A122C, C137T, G144C, C155T, G213A, C279A, G334A and G460A] were identified and located in intron 2, revealing high genetic variability. The alignment of nucleotide sequences was found to be very similar to those for other bovid species. Sequencing and HaeIII digestion showed that frequency of alleles C and A [consisting of fragments of sizes 56, 64, 228, 264, 282, 298 and 497 bp (CC genotype)] was 0.96 and 0.04 for the SNP C279A. Moreover, the genotype frequency of the SNP C279A in gayal was compared with that in other ruminants and it appears that this polymorphism may be associated with low fat content and rapid growth in this rare species.