Dynamic layer-by-layer self-assembly of multi-walled carbon nanotubes on quartz wool for on-line separation of lysozyme in egg white

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• 作者：Zhuo Du ; ^{duzh@mail.sysu.edu.cn} ; Suling Zhang ; Chanyuan Zhou ; Miao Liu ; Gongke Li ; ^{cesgkl@mail.sysu.edu.cn}
• 关键词：Dynamic layer-by-layer self-assembly ; Multi-walled carbon nanotubes ; Quartz wool ; Solid-phase extraction ; Lysozyme
• 刊名：Talanta
• 出版年：2012
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：30 May, 2012
• 卷：94
• 期：Complete
• 页码：104-110
• 文件大小：800 K

摘要

The multi-walled carbon nanotubes (MWNTs) coated quartz wool (MWNTs/QW) prepared by dynamic layer-by-layer self-assembly was used as solid-phase extraction (SPE) absorbent for on-line separation and preconcentration of lysozyme in egg white. The coating procedures were performed continuously in a flow system operated by a set of sequential injection devices. The quartz wool was placed in a microcolumn forming a loose packing to guarantee the minimized flow impedance and the intimate contact between proteins and absorbent surface. Various parameters affecting SPE efficiency including the volume, pH, ionic strength and flow rate of sample and eluent were systematically studied. The feasibility of the proposed method was validated by successfully applied to the separation of lysozyme in egg white.