Functional and genomic analyses of FOXP3-transduced Jurkat-T cells as regulatory T (Treg)-like cells
- 作者：Joon-Young Kim ; Han-Jong Kim ; Elaine M. Hurt ; Xin Chen ; O.M. Zack Howard ; William L. Farrar
- 关键词：FOXP3 ; Treg ; Transduction ; Jurkat-T cells
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：12 October 2007
- 卷：362
- 期：1
- 页码：44-50
- 文件大小：437 K
摘要
FOXP3, a forkhead transcription factor is essential for the development and function of CD4+CD25+ regulatory T cells (Tregs). In contrast to conversion of murine naive T cells to Tregs by transduction of Foxp3, it is controversial whether ectopic expression of FOXP3 in human CD4+ T cells is sufficient for acquisition of suppressive activity. Here, we show that retroviral transduction of FOXP3 induces a Treg phenotype in human leukemic CD4+ Jurkat-T cells, evidenced by increased expression of Treg-associated cell surface markers as well as inhibition of cytokine production. Furthermore, FOXP3-transduced Jurkat-T cells suppress the proliferation of human CD4+CD25− T cells. Additionally, DNA microarray analysis identifies Treg-related genes regulated by FOXP3. Our study demonstrates that enforced expression of FOXP3 confers Treg-like properties on Jurkat-T cells, which can be a convenient and efficient Treg-like cell model for further study to identify Treg cell surface markers and target genes regulated by FOXP3.