Characterization and expression analysis of AcSERK2, a somatic embryogenesis and stress resistance related gene in pineapple

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• 作者：Jun Ma^a ; ^b ; ^{junma365@hotmail.com} ; Yehua He^a ; ^{heyehua@hotmail.com} ; Zhongyi Hu^a ; Wentian Xu^a ; Jingxian Xia^a ; Cuihong Guo^a ; Shunquan Lin^a ; Li Cao^a ; Chengjie Chen^a ; Chenghou Wu^a ; Junli Zhang^b
• 关键词：SERK ; somatic embryogenesis receptor-like kinase ; 2 ; 4-D ; 2 ; 4-dichlorophenoxyacetic acid ; SE ; somatic embryogenesis ; LEC ; Leafy cotyledon ; BBM ; Baby Boom ; AGL15 ; AGAMOUS-like 15 ; WU ; WUSCHEL ; LRR-RLKs ; Leu-rich repeat ; receptor-like kinases ; LRRs ; Leu-rich
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：25 May, 2012
• 卷：500
• 期：1
• 页码：115-123
• 文件大小：1148 K

摘要

The isolation and characterization of m>AcSERK2m>, another somatic embryogenesis receptor-like kinase (SERK) gene homolog, confirmed that there was a m>SERKm> gene family in pineapple. AcSERK2 shared high similarity to AcSERK1 and other SERKs, containing all the characteristic domains of the SERK family. The expressed recombinant protein of pET-m>AcSERK2m> was soluble. m>AcSERK2m> expression was effectively induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and maintained in high level at the early stage of somatic embryogenesis (SE). The results of in situ hybridization showed that m>AcSERK2m> expressed at high level in the competent cells under the induction of 2, 4-D and at low level during the development of meristematic center to globular embryo. This indicated that m>AcSERK2m> could be used as a potential marker gene to monitor the acquisition of embryogenic competence. However, m>AcSERK2m> expression was also detected in all the tested organs and the expression level decreased along with the development of calyx, ovule and ovary. These results suggested that m>AcSERK2m> maybe play a broader role in organ development. m>AcSERK2m> transcription could be induced by exogenous hormones and abiotic stress. It was deduced that m>AcSERK2m> may participate in response to exogenous hormones and abiotic stress regulation in pineapple. The expression pattern of m>AcSERK2m> during SE differed from that of m>AcSERK1m>, and this indicated that the SERK genes may be non-redundant in somatic embryogenesis.