Microsatellite fingerprinting of homonymous grapevine (Vitis vinifera L.) varieties in neighboring regions of South-East Turkey
- 作者：Hü ; seyin Karataş ; Dilek Değ ; irmenci ; Riccardo Velasco ; Silvia Vezzulli ; Ç ; ağ ; rı ; Bodur ; Y. Sabit Ağ ; aoğ ; lu
- 关键词：Vitis vinifera L. ; Microsatellite ; Turkish grapevine germplasm
- 刊名：Scientia Horticulturae
- 出版年：2007
- 期刊代码：93_03044238
- 类别：abs
- 出版时间：1 November 2007
- 卷：114
- 期：3
- 页码：164-169
- 文件大小：298 K
摘要
Genotyping of Turkish grapevine (Vitis vinifera L.) germplasm was characterized by use of six highly polymorphic microsatellite loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79). In this study we aimed to clarify the relationships between homonymous varieties coming from different regions. Our results showed a large degree of genetic variability among most of the homonymous cultivars. The number of alleles per locus ranged from 10 to 21, and gene diversity (expected heterozygosity) values ranged from 0.85 to 0.93. Cultivars presenting the same names of Sergi karası (sampled from Şanlıurfa and Gaziantep), Yediveren (sampled from Şanlıurfa, Gaziantep, and National Germplasm Repository Vineyard in Tekirdağ) and Serpenekıran (sampled from Şanlıurfa and Gaziantep) were clustered together, or very close to each other, in a phenogram. Moreover, the alleles at the six microsatellite loci analyzed were found to be similar in terms of base pairs within each of these three closely positioned varieties. However, all the other cultivars failed to show a suitable clustering pattern when comparing their DNA profiles and names. Similarly named cultivars were not generally grouped together in the phenogram. On the other hand, we detected a tendency for differently named homonymous grape cultivars to cluster together.