The effect of
17x3b2;-estradiol on the secondary damage following spinal cord injury (SCI) was examined in male rats subjected to moderate compression. Two doses of
17x3b2;-estradiol (0.1 or 4 mg/kg) were injected i.p. immediately after spinal cord compression. Functional outcome was observed during 4 weeks following injury with two different tests. Release of cytokines (IL-1
, IL-1
x3b2; and IL-6) was assessed 6 h, 3 days and 1 week post-injury. Reactive astrocytes expressing the glial fibrillary acidic protein GFAP and vimentin, and diffusion of CD68-positive inflammatory cells were examined from 3 days to 4 weeks following SCI. Treatment with
17x3b2;-estradiol significantly increased locomotor function from the first week until 4 weeks post-SCI. The injured spinal cord of
17x3b2;-estradiol-treated rats expressed more IL-1
, IL-1
x3b2; and IL-6 than controls 6 h after injury. Moreover,
17x3b2;-estradiol-treated rats showed reactive astrocytes as soon as 3 days following SCI, with increased GFAP expression, smaller lesion areas and more limited diffusion of CD68-positive cells after 1 week post-injury compared to controls. The number of CD68-positive cells was also reduced in
17x3b2;-estradiol-treated rats one week post-SCI. However, these differences between
17x3b2;-estradiol-treated and control rats disappeared after 4 weeks. These results suggest that
17x3b2;-estradiol protects the spinal cord by stimulating early cytokines release and astroglial responses. These stimulations may prevent the area of damage from expanding and inflammatory cells to spread in the surrounding tissue during the critical first week following SCI. Although transient, these effects improved the locomotor recovery that was sustained over 4 weeks after injury.