A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts

详细信息	查看全文 | 推荐本文 |

• 作者：Eva Ondrouš ; kov&#xe1 ; Karol&#xed ; na Povoln&#xe1 ; Petr V&#xe1 ; ň ; a ; Petr Beneš ; Hana Koneč ; n&#xe1 ; Zbyně ; k Zdr&#xe1 ; hal ; Jan Š ; marda
• 关键词：v-myb ; Monoblast ; Macrophage ; Differentiation ; Proteome ; Two-dimensional gel electrophoresis ; Trichostatin A ; TPA ; Mass spectrometry
• 刊名：Leukemia Research
• 出版年：2007
• 期刊代码：186_01452126
• 类别：med
• 出版时间：February 2007
• 卷：31
• 期：2
• 页码：221-229
• 文件大小：1192 K

摘要

v-myb oncogene of avian myeloblastosis virus (AMV) transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation.