The hydrophobic segment of Arabidopsis thaliana cluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes

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• 作者：Marie-No&#xeb ; lle Vaultier ; Catherine Cantrel ; Fran&#xe7 ; oise Guerbette ; Yohann Boutt&#xe9 ; Chantal Vergnolle ; Dominique Ç ; i&#xe7 ; ek ; Susanne Bolte ; Alain Zachowski ; Eric Ruell
• 关键词：Diacylglycerol kinases ; Integral protein ; Hydrophobic domain ; GFP ; Membrane sequestering ; <i>Arabidopsis thalianai>
• 刊名：FEBS Letters
• 出版年：2008
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：28 May 2008
• 卷：582
• 期：12
• 页码：1743-1748
• 文件大小：796 K

摘要

Diacylglycerol kinases (DGKs) catalyze the phosphorylation of diacylglycerol into phosphatidic acid. To fulfill their role in many signalling processes, DGKs must be located at, or in, membranes. Most mammalian DGKs are cytosolic and are recruited to membranes upon stimulation, except for <i>εi> type DGKs that are permanently membrane-associated through a hydrophobic segment. Nothing is known about the mechanism(s) involved in the membrane localization of plant DGKs. By fusion to fluorescent proteins, we show that two DGKs from cluster I in <i>Arabidopsis thalianai> possess amino-terminal hydrophobic segments that are sufficient to address them to endoplasmic reticulum membranes.