Structural analysis of the α-<typestyle value="smcaps">d-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

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• 作者：S ; er S. van Leeuwen ; Slavko Kralj ; Ineke H. van Geel-Schutten ; Gerrit J. Gerwig ; Lubbert Dijkhuizen ; Johannis P. Kamerling
• 关键词：ttp ; //www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0">-d-Glucan ; Polysaccharide structural analysis ; GTF180 Glucansucrase ; ¹H N
• 刊名：Carbohydrate Research
• 出版年：2008
• 期刊代码：15_00086215
• 类别：ch
• 出版时间：19 May 2008
• 卷：343
• 期：7
• 页码：1237-1250
• 文件大小：447 K

摘要

The neutral exopolysaccharide trong>EPS180trong> produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-ttp://www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0">-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D ¹H and ¹³C NMR spectroscopy of the intact trong>EPS180trong>, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of trong>EPS180trong>, a composite model, that includes all identified structural features, was formulated as follows: