Isolation and characterisation of neural progenitor cells from the adult Chx10^orJ/orJ central neural retina

详细信息	查看全文 | 推荐本文 |

• 作者：I. Kokkinopoulos ; R.A. Pearson ; A. MacNeil ; N.S. Dhomen ; R.E. MacLaren ; R.R. Ali ; J.C. Sowden
• 关键词：Adult stem cell ; Neural progenitor cell ; Mouse ; Neurosphere ; Chx10 ; Retina ; M&#xfc ; ller cell ; M&#xfc ; ller progenitor
• 刊名：Molecular and Cellular Neuroscience
• 出版年：2008
• 期刊代码：173_10447431
• 类别：bio
• 出版时间：July 2008
• 卷：38
• 期：3
• 页码：359-373
• 文件大小：6812 K

摘要

Retinal stem cells have been isolated from the ciliary epithelium (CE) of the mammalian retina. However, the central neural retina (CNR) lacks the capability to regenerate, a phenomenon retained by lower vertebrates. Mutations in the Chx10 homeobox gene cause reduced proliferation of retinal progenitor cells during development, leading to microphthalmia. Recently, we showed that in Chx10^orJ/orJ mice, dividing cells persist in the adult CNR, suggesting the existence of a dormant progenitor population. Here, we show that these cells are proliferative and give rise to neurospheres in vitro, a characteristic of neural stem cells. However, these adult-derived CNR progenitors differ from those of the wildtype CE, leading to de-pigmented, larger and more numerous neurospheres expressing Müller glial cell markers. Our results suggest that lack of Chx10 leads to maintenance of a dormant neural progenitor population in the adult CNR. Furthermore, Chx10 is not required for in vitro proliferation of these progenitors.