Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens
- 作者：Xiaobang Hu ; Weijie Wang ; Donghui Zhang ; Jianhua Jiao ; Wenbin Tan ; Yan Sun ; Lei Ma ; Changliang Zhu
- 关键词：Culex pipiens pallens ; Deltamethrin resistance ; Developmental expression ; Real-time PCR ; Ribosomal protein
- 刊名：Comparative Biochemistry and Physiology ; Part B
- 出版年：2007
- 期刊代码：50_10964959
- 类别：bio
- 出版时间：February 2007
- 卷：146
- 期：2
- 页码：265-270
- 文件大小：714 K
摘要
The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96%and 91%identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.