Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens
- 作者：Xiaobang Hu ; Yan Sun ; Weijie Wang ; Mingxia Yang ; Lixin Sun ; Wenbin Tan ; Jing Sun ; Jin Qian ; Lei Ma ; Donghui Zhang ; et al.
- 关键词：Culex pipiens pallens ; Opsin ; Reverse transcription PCR ; Transfection ; Deltamethrin resistance
- 刊名：Pesticide Biochemistry and Physiology
- 出版年：2007
- 期刊代码：182_00483575
- 类别：bio
- 出版时间：May 2007
- 卷：88
- 期：1
- 页码：82-91
- 文件大小：2234 K
摘要
One mosquito opsin gene, NYD-OP7, has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 1116 bp was found to encode a putative 371 amino acids protein which exhibits high identity with opsins from Aedes and Anopheles mosquitoes. Transcript expression of NYD-OP7 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains of the Culex mosquito. The results demonstrated that this gene is expressed at all developmental stages, and it is expressed predominantly at the pupae and adult stages. Meanwhile, in pupae and adults, NYD-OP7 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Importantly, stable expression of NYD-OP7 in the mosquito C6/36 cells can confer moderate deltamethrin resistance. Our study provides the first direct evidence that increased expression of an opsin gene may play some role in the development of deltamethrin resistance in Cx. pipiens pallens.