Most MCF7 and SK-OV3 cells were deprived of their stem nature by Hoechst 33342
- 作者：Yong Zhong ; Chunxia Zhou ; Wenbo Ma ; Dongmei Wang ; Sujuan Guo ; Xiaosan Su ; Shuren Zhang
- 关键词：Cancer stem cell ; Side population ; Hoechst 33342 ; MCF7 ; SK-OV3
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：14 December 2007
- 卷：364
- 期：2
- 页码：338-343
- 文件大小：1612 K
摘要
Using clonal and population analysis, we found that the MCF7 and SK-OV3 were composed mainly of cancer stem cells. Together with SP analysis, we found that both SP and NSP cells of MCF7 and SK-OV3 contained different proportions of cancer stem cells. In addition, we discovered that MCF7 SP cells were able to generate non-SP cells, and conversely non-SP cells generated SP cells; Moreover, we showed that Hoechst 33342 and FACS were harmful to the clonogenicity of MCF7 and SK-OV3 cells.