Primary style protein expression in the self-incompatible/compatible apricot by the 2D-DIGE technique
- 作者：Xiaoyan Cao caoxiaoyan823@126.com ; Jianrong Feng ; fengjr102@gmail.com ; Dajiang Wang ; Junli Sun ; Xiaoyan Lu ; Huaifeng Liu
- 关键词：SI ; Self-incompatibility ; SC ; Self-compatibility ; 2D-DIGE ; Two dimensional fluorescence difference gel electrophoresis ; MS ; Mass spectrum ; IM ; Intercellular matrix ; 2-DE ; Two-dimensional electrophoresis ; LC-MS/MS ; Liquid chromatography&ndash ; tandem mass s
- 刊名：Gene
- 出版年：2012
- 期刊代码：73_03781119
- 类别：bio
- 出版时间：15 July, 2012
- 卷：503
- 期：1
- 页码：110-117
- 文件大小：1438 K
摘要
In order to explore the molecular mechanism underlying self-incompatibility (SI) in the apricot (Prunus armeniaca L.) at the proteome level, we examined the style proteomes at different stages of flower development: small bud, big bud, 24 h after self-pollination and 24 h after cross-pollination with cultivar Badanshui in the SI apricot cultivar Xinshiji and the self-compatible (SC) apricot cultivar Katy by 2D fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). About 1500 style protein spots were detected; 66 were expressed differently in the four stages in Xinshiji. About 1600 style protein spots were detected; 143 were expressed differently in the four stages of flower development in Katy. In Xinshiji, one protein was expressed specifically, four proteins showed up-regulated expression and twenty-nine proteins showed down-regulated expression in the cross-pollinated style compared to the self-pollinated style. Thirteen proteins were identified unambiguously. In Katy, three proteins were expressed specifically, five proteins showed up-regulated expression and thirteen proteins showed down-regulated expression in the cross-pollinated style compared to self-pollinated style. Seven proteins were identified unambiguously. The different reactions of the style at the proteomic level were triggered in Xinshiji and Katy by self pollen and non-self pollen.