MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma
- 作者：Xiaohui Xu ; Zhaoli Chen ; Xiaohong Zhao ; Jiwen Wang ; Dapeng Ding ; Zhen Wang ; Fengwei Tan ; Xiaogang Tan ; Fang Zhou ; Jian Sun ; Nan Sun ; Yibo Gao ; Kang Shao ; Ning Li ; Bin Qiu ; Jie He ; prof.hejie@263.net
- 关键词：MicroRNA ; Esophageal squamous cell carcinoma ; Cell migration ; Cell invasion ; CDH1
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：18 May, 2012
- 卷：421
- 期：4
- 页码：640-645
- 文件大小：1070 K
摘要
MicroRNAs (miRNAs) as a species of small non coding single stranded RNA of about 21-25 nucleotides have important roles in the development of different cancers. In present study, we found that the expression of miR-25 was up-regulated in 60 esophageal squamous cell carcinoma (ESCC) tissues compared with matched adjacent non-cancer tissues. Moreover, we demonstrated that the up-regulation of miR-25 was significantly correlated with the status of lymph node metastasis and TNM (Tumor, Node and Metastasis) stage. Furthermore, over-expression of miR-25 markedly promoted migration and invasion of ESCC cells. On the contrary, down-regulation of miR-25 inhibited the migration and invasion of cells. E-cadherin(CDH1) is a very important tumor metastasis suppressor. We further identified that miR-25 directly targeted CDH1 3鈥?untranslated region (3鈥睻TR) and repressed the expression of CDH1. These results, for the first time, demonstrate that miR-25 promotes ESCC cell migration and invasion by suppressing CDH1 expression.