Real-time PCR quantification of the population dynamics of ammonia-oxidizing bacteria in a pilot-scale wastewater treatment plant

详细信息	查看全文 | 推荐本文 |

• 作者：Xiaohui Wang ; Ya Zhang ; Xianghua Wen ; ^{xhwen@tsinghua.edu.cn} ; Yu Xia ; Man Hu ; Zhaofang ; Kun Ding
• 关键词：Waste water treatment ; Biodegradation ; Bioreactors ; DNA ; AOB population dynamics ; Real-time PCR
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 July, 2012
• 卷：66
• 期：Complete
• 页码：61-65
• 文件大小：343 K

摘要

To determine whether the stable nitrification was correlated with a stable AOB population size in a pilot-scale wastewater treatment plant (WWTP) and to relate the AOB population to the ammonia removal efficiency, the population dynamics of AOB in a pilot-scale WWTP was monitored over a half-year period using real-time PCR assay. During the study period, the effluent ammonia concentrations were below 2.5 mg/L almost all the time, indicting a stable function of nitrification. The change of AOB population was relatively stable, ranging from 0.7 脳 10¹⁰ to 7.9 脳 10¹⁰ cells per liter mixed liquor suspended solids (MLSS) mixture, indicating that the stable function of nitrification was correlated with a stable AOB number in the pilot-scale WWTP. Correlation analysis demonstrates a significantly positive correlation between ammonia removal efficiency and total AOB population number (r = 0.711, P < 0.01). The findings enrich the theory involving the relation between AOB population dynamics and function of nitrification in WWTPs.