Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China
- 作者：Jiansheng Wang wangjiansheng_17@yahoo.com.cn ; Honghui Gu ; gu2199@yahoo.com.cn ; Huifang Yu ; Zhenqing Zhao ; Xiaoguang Sheng ; Xiaohui Zhang
- 关键词：Broccoli ; Glucosinolate ; Sulforaphane ; Anti-cancer
- 刊名：Food Chemistry
- 出版年：2012
- 期刊代码：48_03088146
- 类别：abs
- 出版时间：1 August, 2012
- 卷：133
- 期：3
- 页码：735-741
- 文件大小：471 K
摘要
Five main cultivars from China and 143 parent materials grown in a greenhouse were used to investigate the glucosinolates in broccoli florets. Eight aliphatic glucosinolates, four indole glucosinolates, and one aromatic glucosinolate were identified and quantified using high-performance liquid chromatography (HPLC). The results showed that glucoraphanin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrassicin were present in all samples. However, the predominant type of glucosinolate was different among pure lines. The anti-cancer glucoraphanin concentration ranged from 0.06 to 24.17 渭mol/g in pure lines and from 1.57 to 5.95 渭mol/g in commercial cultivars. The progoitrin concentration in commercial cultivars varied from 1.77 to 6.07 渭mol/g with a mean value of 3.20 渭mol/g. Significant variations were observed in the concentration of individual glucosinolates and in each class of glucosinolates among broccoli populations. Dozens of specific lines with altered glucosinolate profiles, as well as ten good candidates for breeding high-chemoprotective glucosinolate cultivars, were obtained according to the putative glucosinolate pathway in broccoli.