Media optimization for elevated molecular weight and mass production of pigment-free pullulan

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• 作者：Xiaoliu Yu ; Yulei Wang ; Gongyuan Wei ; ^{weigy@suda.edu.cn} ; Yingying Dong
• 关键词：Pullulan ; Molecular weight ; Orthogonal array design ; Response surface methodology ; Aureobasidium pullulans
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 July, 2012
• 卷：89
• 期：3
• 页码：928-934
• 文件大小：494 K

摘要

In this study, an Aureobasidium pullulans SZU 1001 mutant, deficient in pigment production, was screened by complex UV and 纬-ray mutagenesis. Medium composition optimization for increased pullulan molecular weight and production was conducted using this mutant. Six nutrients: yeast extract, (NH₄)₂SO₄, K₂HPO₄, NaCl, MgSO₄路7H₂O and CaCl₂ were detected within pullulan production in flasks. It is shown that NaCl and K₂HPO₄ have significant influences on molecular weight of pullulan, while yeast extract and (NH₄)₂SO₄ significantly affect pullulan yield. To achieve a higher molecular weight and more efficient pullulan production, a response surface methodology approach was introduced to predict an optimal nutrient combination. A molecular weight of 5.74 脳 10⁶ and pullulan yield on glucose of 51.30%were obtained under batch pullulan fermentation with the optimized media, which increased molecular weight and pullulan production by 97.25%and 11.04%, respectively compared with the control media.